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KMID : 0545120190290081310
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Journal of Microbiology and Biotechnology
2019 Volume.29 No. 8 p.1310 ~ p.1315
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Development of a New Type of Recombinant Hyaluronidase Using a Hexahistidine; Possibilities and Challenges in Commercialization
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Park Chae-Ri
Song Yun-Kyoung
Ki Young-Hyun
Jung Ye-Na
Park Young-Ho
Song Bong-Seok
Eom Tae-Kil
Kim Ju-Sung
Kim Sang-Hyun
Kim Ji-Su
Kim Sun-Uk
Lee Sang-Rae
Kim E-Kyune
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Abstract
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Hyaluronidases enhance therapeutic drug transport by breaking down the hyaluronan barrier to lymphatic and capillary vessels, facilitating their tissue absorption. Commercially available hyaluronidases are bovine in origin; however, they pose risks such as bovine spongiform encephalopathy. The present study aimed to develop a novel, highly active hyaluronidase and assess its function. Therefore, in order to find the most efficient active hyaluronidase, we produced several shortened hyaluronidases with partial removal of the N- or C-terminal regions. Moreover, we created an enzyme that connected six histidines onto the end of the hyaluronidase C-terminus. This simplified subsequent purification using Ni2+ affinity chromatography, making it feasible to industrialize this highly active recombinant hyaluronidase which exhibited catalytic activity equal to that of the commercial enzyme. Therefore, this simple and effective isolation method could increase the availability of recombinant hyaluronidase for research and clinical purposes.
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KEYWORD
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Recombinant, hyaluronidase, affinity chromatography, enzyme, hyaluronic acid
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